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Synthego Inc grna sgrna sequences flanking ugp2 exon 2
Grna Sgrna Sequences Flanking Ugp2 Exon 2, supplied by Synthego Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/grna-2+sequence/sgrna/pm40769148-755-20-30
Average 86 stars, based on 1 article reviews
grna sgrna sequences flanking ugp2 exon 2 - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Knock-Out:

Article Title: Increased translation driven by non-canonical EZH2 creates a synthetic vulnerability in enzalutamide-resistant prostate cancer
Article Snippet: .. To knock-out Prkci in Pten f/f Trp53 f/f Rb1 f/f PbCre + organoids, a single-guide RNA sequence targeting Prkci exon 2 was purchased from Synthego (Supplementary Table ) and transduced with recombinant Streptococcus pyogenes Cas9 protein (Thermo Fisher Scientific, cat No. A36498), using the Neon Transfection System 1 (Invitrogen) following the manufacturer’s protocol and single clones were expanded and screened by immunoblotting. .. LNCaP and C4-2B cells were cultured in Rosewell Park Memorial Institute 1640 (RPMI 1640) (CORNING, cat No. 15-040-CV).

Sequencing:

Article Title: Increased translation driven by non-canonical EZH2 creates a synthetic vulnerability in enzalutamide-resistant prostate cancer
Article Snippet: .. To knock-out Prkci in Pten f/f Trp53 f/f Rb1 f/f PbCre + organoids, a single-guide RNA sequence targeting Prkci exon 2 was purchased from Synthego (Supplementary Table ) and transduced with recombinant Streptococcus pyogenes Cas9 protein (Thermo Fisher Scientific, cat No. A36498), using the Neon Transfection System 1 (Invitrogen) following the manufacturer’s protocol and single clones were expanded and screened by immunoblotting. .. LNCaP and C4-2B cells were cultured in Rosewell Park Memorial Institute 1640 (RPMI 1640) (CORNING, cat No. 15-040-CV).

Article Title: Tau uptake by human neurons depends on receptor LRP1 and kinase LRRK2
Article Snippet: .. LRRK2-targeting gRNA sequence: ATTGCAAAGATTGCTGACTA , Synthego , . ..

Article Title: TIGIT contributes to the regulation of 4-1BB and does not define NK cell dysfunction in glioblastoma
Article Snippet: gRNA sequence 1: UCUUCCCUAGGAAUGAUGAC , Synthego , N/A. .. gRNA sequence 2: AUGUCACCUCUCCUCCACCA , Synthego , N/A. .. gRNA sequence 3: GGCCAUUUGUAAUGCUGACU , Synthego , N/A.

Article Title: Combined PD-L1/TGFβ blockade allows expansion and differentiation of stem cell-like CD8 T cells in immune excluded tumors.
Article Snippet: .. EMT6 IFNGR1 KO cells were generated at Synthego (Redwood City, CA) using CRISPR KO (Guide RNA sequence: UGGUAUUCCCAGCAUACGAC; Guide RNA cut location chr10:19,597,466; Exon targeted: 2). .. The loss of IFNγ response in the EMT6 IFNGR1 KO cells was validated in vitro by stimulating the cells with mouse recombinant IFNγ Nature Communications | (2023) 14:4703 11 (catalog number n 485-MI, R&D Systems,Minneapolis, MN) for 30min at 37 °C and by analyzing STAT1 phosphorylation by flow cytometry.

Transduction:

Article Title: Increased translation driven by non-canonical EZH2 creates a synthetic vulnerability in enzalutamide-resistant prostate cancer
Article Snippet: .. To knock-out Prkci in Pten f/f Trp53 f/f Rb1 f/f PbCre + organoids, a single-guide RNA sequence targeting Prkci exon 2 was purchased from Synthego (Supplementary Table ) and transduced with recombinant Streptococcus pyogenes Cas9 protein (Thermo Fisher Scientific, cat No. A36498), using the Neon Transfection System 1 (Invitrogen) following the manufacturer’s protocol and single clones were expanded and screened by immunoblotting. .. LNCaP and C4-2B cells were cultured in Rosewell Park Memorial Institute 1640 (RPMI 1640) (CORNING, cat No. 15-040-CV).

Recombinant:

Article Title: Increased translation driven by non-canonical EZH2 creates a synthetic vulnerability in enzalutamide-resistant prostate cancer
Article Snippet: .. To knock-out Prkci in Pten f/f Trp53 f/f Rb1 f/f PbCre + organoids, a single-guide RNA sequence targeting Prkci exon 2 was purchased from Synthego (Supplementary Table ) and transduced with recombinant Streptococcus pyogenes Cas9 protein (Thermo Fisher Scientific, cat No. A36498), using the Neon Transfection System 1 (Invitrogen) following the manufacturer’s protocol and single clones were expanded and screened by immunoblotting. .. LNCaP and C4-2B cells were cultured in Rosewell Park Memorial Institute 1640 (RPMI 1640) (CORNING, cat No. 15-040-CV).

Transfection:

Article Title: Increased translation driven by non-canonical EZH2 creates a synthetic vulnerability in enzalutamide-resistant prostate cancer
Article Snippet: .. To knock-out Prkci in Pten f/f Trp53 f/f Rb1 f/f PbCre + organoids, a single-guide RNA sequence targeting Prkci exon 2 was purchased from Synthego (Supplementary Table ) and transduced with recombinant Streptococcus pyogenes Cas9 protein (Thermo Fisher Scientific, cat No. A36498), using the Neon Transfection System 1 (Invitrogen) following the manufacturer’s protocol and single clones were expanded and screened by immunoblotting. .. LNCaP and C4-2B cells were cultured in Rosewell Park Memorial Institute 1640 (RPMI 1640) (CORNING, cat No. 15-040-CV).

Clone Assay:

Article Title: Increased translation driven by non-canonical EZH2 creates a synthetic vulnerability in enzalutamide-resistant prostate cancer
Article Snippet: .. To knock-out Prkci in Pten f/f Trp53 f/f Rb1 f/f PbCre + organoids, a single-guide RNA sequence targeting Prkci exon 2 was purchased from Synthego (Supplementary Table ) and transduced with recombinant Streptococcus pyogenes Cas9 protein (Thermo Fisher Scientific, cat No. A36498), using the Neon Transfection System 1 (Invitrogen) following the manufacturer’s protocol and single clones were expanded and screened by immunoblotting. .. LNCaP and C4-2B cells were cultured in Rosewell Park Memorial Institute 1640 (RPMI 1640) (CORNING, cat No. 15-040-CV).

Western Blot:

Article Title: Increased translation driven by non-canonical EZH2 creates a synthetic vulnerability in enzalutamide-resistant prostate cancer
Article Snippet: .. To knock-out Prkci in Pten f/f Trp53 f/f Rb1 f/f PbCre + organoids, a single-guide RNA sequence targeting Prkci exon 2 was purchased from Synthego (Supplementary Table ) and transduced with recombinant Streptococcus pyogenes Cas9 protein (Thermo Fisher Scientific, cat No. A36498), using the Neon Transfection System 1 (Invitrogen) following the manufacturer’s protocol and single clones were expanded and screened by immunoblotting. .. LNCaP and C4-2B cells were cultured in Rosewell Park Memorial Institute 1640 (RPMI 1640) (CORNING, cat No. 15-040-CV).

Generated:

Article Title: Combined PD-L1/TGFβ blockade allows expansion and differentiation of stem cell-like CD8 T cells in immune excluded tumors.
Article Snippet: .. EMT6 IFNGR1 KO cells were generated at Synthego (Redwood City, CA) using CRISPR KO (Guide RNA sequence: UGGUAUUCCCAGCAUACGAC; Guide RNA cut location chr10:19,597,466; Exon targeted: 2). .. The loss of IFNγ response in the EMT6 IFNGR1 KO cells was validated in vitro by stimulating the cells with mouse recombinant IFNγ Nature Communications | (2023) 14:4703 11 (catalog number n 485-MI, R&D Systems,Minneapolis, MN) for 30min at 37 °C and by analyzing STAT1 phosphorylation by flow cytometry.

CRISPR:

Article Title: Combined PD-L1/TGFβ blockade allows expansion and differentiation of stem cell-like CD8 T cells in immune excluded tumors.
Article Snippet: .. EMT6 IFNGR1 KO cells were generated at Synthego (Redwood City, CA) using CRISPR KO (Guide RNA sequence: UGGUAUUCCCAGCAUACGAC; Guide RNA cut location chr10:19,597,466; Exon targeted: 2). .. The loss of IFNγ response in the EMT6 IFNGR1 KO cells was validated in vitro by stimulating the cells with mouse recombinant IFNγ Nature Communications | (2023) 14:4703 11 (catalog number n 485-MI, R&D Systems,Minneapolis, MN) for 30min at 37 °C and by analyzing STAT1 phosphorylation by flow cytometry.



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Image Search Results


Journal: iScience

Article Title: TIGIT contributes to the regulation of 4-1BB and does not define NK cell dysfunction in glioblastoma

doi: 10.1016/j.isci.2023.108353

Figure Lengend Snippet:

Article Snippet: gRNA sequence 2: AUGUCACCUCUCCUCCACCA , Synthego , N/A.

Techniques: Recombinant, Lactate Dehydrogenase Assay, Cell Based Assay, Gene Knockout, Enzyme-linked Immunosorbent Assay, shRNA, Sequencing, Software, Gene Expression

Plasmids used in this work

Journal: Microbial Cell Factories

Article Title: Development of an industrial yeast strain for efficient production of 2,3-butanediol

doi: 10.1186/s12934-022-01924-z

Figure Lengend Snippet: Plasmids used in this work

Article Snippet: P58-PDC1 , P58 backbone with 2 gRNA targeting sequence for PDC1 (AGCATCCAACAATTTTTGCA and GATAAGCTTTATGAAGTCAA) , MCB, KU Leuven.

Techniques: Marker, Plasmid Preparation, Sequencing, Expressing